Johan Dahlin
81790199af
All checks were successful
Deploy Quartz site to GitHub Pages / build (push) Successful in 2m4s
24 lines
1.3 KiB
Markdown
24 lines
1.3 KiB
Markdown
---
|
||
tags:
|
||
- biokemi
|
||
- instuderingsuppgifter
|
||
- plasmid
|
||
---
|
||
#### Name three applications of molecular cloning.
|
||
#### What is a plasmid?
|
||
#### Name the three main steps involved in recombinant DNA technology?
|
||
#### What is a restriction enzyme?
|
||
#### What are the bacteria used in your protocol?
|
||
#### Explain the calcium/phosphate (heat-shock) method and what it is used for.
|
||
#### How can we selectively grow bacteria that have taken up the plasmid?
|
||
#### Explain the principle behind blue and white screening and its purpose in this lab.
|
||
|
||
#### What are the sites the plasmid contains that allow for this experiment?
|
||
#### What are the 3 compounds in the LB plates that allow for selection of bacteria with plasmid and the distinction of plasmids with and without the inserted gene?
|
||
#### What are the six steps in plasmid preparation and purification?
|
||
#### What is a restriction enzyme and why was it used in this experiment?
|
||
#### Explain the principle of Gel Electrophoresis, what is it for?
|
||
#### What´s the compound that allows for the visualization of the DNA in the gel?
|
||
#### How many times does BamHI cut the plasmid without the insert?
|
||
#### And the plasmid with the insert?
|
||
#### Explain the different plasmid conformations that exist. Förståelse Biokemi / Plasmidlabb / |