---
tags:
  - biokemi
  - instuderingsuppgifter
  - plasmid
---
#### Name three applications of molecular cloning. 
#### What is a plasmid? 
#### Name the three main steps involved in recombinant DNA technology? 
#### What is a restriction enzyme? 
#### What are the bacteria used in your protocol? 
#### Explain the calcium/phosphate (heat-shock) method and what it is used for. 
#### How can we selectively grow bacteria that have taken up the plasmid? 
#### Explain the principle behind blue and white screening and its purpose in this lab.

#### What are the sites the plasmid contains that allow for this experiment? 
#### What are the 3 compounds in the LB plates that allow for selection of bacteria with plasmid and the distinction of plasmids with and without the inserted gene?
#### What are the six steps in plasmid preparation and purification?
#### What is a restriction enzyme and why was it used in this experiment? 
#### Explain the principle of Gel Electrophoresis, what is it for? 
#### What´s the compound that allows for the visualization of the DNA in the gel? 
#### How many times does BamHI cut the plasmid without the insert? 
#### And the plasmid with the insert? 
#### Explain the different plasmid conformations that exist. Förståelse Biokemi / Plasmidlabb /