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+ <small>The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.</small>
+
+<small>In other words, they studied human hematopoiesis acros s age by analyzing purified blood cell lineages from the subjects.</small>
+
+---
+👋 
+
+-------
+## Study
+- 197 hematologically normal females
+	- 23 newborn - taken during delivery
+	- 94 young adult - age: 17-50 median 30
+	- 80 eldery - age: 75-96 median 81
+- purified neutrofils
+- purified T-cells
+----
+### Methods
+1. <small>Peripheral blood samples were collected</small>
+2. <small>Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_</small>
+3. <small>X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_</small>
+4. <small>T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_</small >