diff --git a/content/.obsidian/plugins/emoji-shortcodes/data.json b/content/.obsidian/plugins/emoji-shortcodes/data.json index 427164e..1b64558 100644 --- a/content/.obsidian/plugins/emoji-shortcodes/data.json +++ b/content/.obsidian/plugins/emoji-shortcodes/data.json @@ -4,6 +4,7 @@ "historyPriority": true, "historyLimit": 100, "history": [ + ":wave:", ":white_check_mark:", ":arrow_up_small:", ":arrow_down_small:", diff --git a/content/.obsidian/plugins/image-converter/image-converter-image-alignments.json b/content/.obsidian/plugins/image-converter/image-converter-image-alignments.json index 1b664d3..6701737 100644 --- a/content/.obsidian/plugins/image-converter/image-converter-image-alignments.json +++ b/content/.obsidian/plugins/image-converter/image-converter-image-alignments.json @@ -1,5 +1,5 @@ { - "Vetenskaplig artikel/Methods & Main Results.md": { + "Vetenskaplig artikel/Intro.md": { "aca677ab589429f9db4b9295c7397939": { "position": "center", "width": "148px", diff --git a/content/Vetenskaplig artikel/Intro.md b/content/Vetenskaplig artikel/Intro.md new file mode 100644 index 0000000..1103675 --- /dev/null +++ b/content/Vetenskaplig artikel/Intro.md @@ -0,0 +1,21 @@ + The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells. + +In other words, they studied human hematopoiesis acros s age by analyzing purified blood cell lineages from the subjects. + +--- +👋 + +------- +## Study +- 197 hematologically normal females + - 23 newborn - taken during delivery + - 94 young adult - age: 17-50 median 30 + - 80 eldery - age: 75-96 median 81 +- purified neutrofils +- purified T-cells +---- +### Methods +1. Peripheral blood samples were collected +2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_ +3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_ +4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_ \ No newline at end of file diff --git a/content/Vetenskaplig artikel/Methods & Main Results.md b/content/Vetenskaplig artikel/Methods & Main Results.md deleted file mode 100644 index 27b23e0..0000000 --- a/content/Vetenskaplig artikel/Methods & Main Results.md +++ /dev/null @@ -1,49 +0,0 @@ -The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells. -In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects. - ------ -## Study -- 1 97 hematologically normal females - - 23 newborn - - 94 young adult (median 30) - - 80 eldery (over 75) -- purified neutrofils -- purified T-cells ----- -### Metoder -1. Peripheral blood samples were collected -2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_ -3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_ -4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_ ---- -### Results -1. X chromosome usage is similar in newborns and young adults. -2. Neutrophils from elderly female individuals show strongly uneven X chromosome usage. -3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion ---- -### Cord blood neutrophils -![[image-183.png|148x156]] - -- Graph shows the distribution of X chromosome usage in 23 newborn -- X-axis: % of cells using the less common X chromosome. -- Y-axis: number of individuals in each group. -Conclusion: Newborns mostly show balanced use of the two X chromosomes - - ---- -### > 75 years neutrophils - -![[image-184.png|145x158]] - -- This graph shows the purified neutrofils from 80 elderly (y >= 75) females -- X and Y axis is the same as in previous graph -Conclusion: Elderly neutrophils frequently show strongly uneven X chromosome usage. - ----- -### Questions -- What was the main question the authors wanted to answer? -- Why did they choose this specific method instead of another one? -- What does this figure show in one sentence? -- What is the main conclusion from your article? -- What would be the biggest limitation of this study? -