Johan Dahlin
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The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.
In other words, they studied human hematopoiesis acros s age by analyzing purified blood cell lineages from the subjects.
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Study
- 197 hematologically normal females
- 23 newborn - taken during delivery
- 94 young adult - age: 17-50 median 30
- 80 eldery - age: 75-96 median 81
- purified neutrofils
- purified T-cells
Methods
- Peripheral blood samples were collected
- Neutrophils and T cells were isolated using cell separation techniques (centrifugation followed by lineage-specific purification)
- X-chromosome inactivation was analyzed using the HUMARA PCR assay (PCR-based measurement of which X chromosome is active)
- T cells were analyzed by PCR to check for clonal expansion (T-cell receptor γ gene rearrangement analysis)