vault backup: 2026-01-16 14:36:49

content/.obsidian/plugins/emoji-shortcodes/data.json

@@ -4,6 +4,7 @@
   "historyPriority": true,
   "historyLimit": 100,
   "history": [
+    ":wave:",
     ":white_check_mark:",
     ":arrow_up_small:",
     ":arrow_down_small:",

content/.obsidian/plugins/image-converter/image-converter-image-alignments.json

@@ -1,5 +1,5 @@
 {
-  "Vetenskaplig artikel/Methods & Main Results.md": {
+  "Vetenskaplig artikel/Intro.md": {
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       "position": "center",
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content/Vetenskaplig artikel/Intro.md

@@ -0,0 +1,21 @@
+ <small>The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.</small>
+
+<small>In other words, they studied human hematopoiesis acros s age by analyzing purified blood cell lineages from the subjects.</small>
+
+---
+👋 
+
+-------
+## Study
+- 197 hematologically normal females
+	- 23 newborn - taken during delivery
+	- 94 young adult - age: 17-50 median 30
+	- 80 eldery - age: 75-96 median 81
+- purified neutrofils
+- purified T-cells
+----
+### Methods
+1. <small>Peripheral blood samples were collected</small>
+2. <small>Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_</small>
+3. <small>X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_</small>
+4. <small>T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_</small >

content/Vetenskaplig artikel/Methods & Main Results.md

@@ -1,49 +0,0 @@
-The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.
-In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects.
-
------
-## Study
-- 1 97 hematologically normal females
-	- 23 newborn
-	- 94 young adult (median 30)
-	- 80 eldery (over 75)
-- purified neutrofils
-- purified T-cells
-----
-### Metoder
-1. Peripheral blood samples were collected
-2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_
-3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_
-4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_
----
-### Results
-1. X chromosome usage is similar in newborns and young adults.
-2. Neutrophils from elderly female individuals show strongly uneven X chromosome usage.
-3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion
----
-### Cord blood neutrophils
-![[image-183.png|148x156]] 
-<small>
-- Graph shows the distribution of X chromosome usage in 23 newborn
-- X-axis: % of cells using the less common X chromosome.
-- Y-axis: number of individuals in each group.
-Conclusion: Newborns mostly show balanced use of the two X chromosomes
-</small>
-
----
-### > 75 years neutrophils
-
-![[image-184.png|145x158]]
-<small>
-- This graph shows the purified neutrofils from 80 elderly (y >= 75) females
-- X and Y axis is the same as in previous graph
-Conclusion: Elderly neutrophils frequently show strongly uneven X chromosome usage.
-</small> 
-----
-### Questions
-- What was the main question the authors wanted to answer?
-- Why did they choose this specific method instead of another one?
-- What does this figure show in one sentence?
-- What is the main conclusion from your article?
-- What would be the biggest limitation of this study?
-