Johan Dahlin
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50 lines
1.6 KiB
Markdown
50 lines
1.6 KiB
Markdown
Modell/material
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- 197 hematologically normal females
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- 23 new born
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- 94 young adult (median 30)
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- 80 eldery (over 75)
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- purified neutrofils
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- purified T-cells
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The model is _human hematopoiesis across age_ and the material is _lineage-purified blood cells_
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methods:
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1. cell separation (neutrofil and T-cell)
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2. x-chromosome inactivation analysis (humana PCR)
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3. T-cell clonality check
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> [!NOTE]
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> They separated neutrophils and T cells, measured X-inactivation skewing, and checked that T-cells were not dominating.
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### **What was compared / tested**
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- **Age groups:** newborn vs young adults vs elderly
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- **Cell lineages:** neutrophils vs T cells
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- **Readout:** degree of X-chromosome inactivation skewing
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### Results
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1. X chromosome usage is similar in newborns and young adults.
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2. Neutrophils from elderly individuals show strongly uneven X chromosome usage.
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3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion
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### Figure
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![[image-183.png|177x186]]
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Notes
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- This graph shows the distribution of X chromosome usage in 23 newborn females
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- X-axis: in % how uneven the X chromosome usage is
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- 50% means perfectly (50% maternal / 50% paternal)
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- 0/100% means all cells use the same chromosome
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- Y-axis: number of individuals in each group
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Conclusion:
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- Newborns mostly show balanced use of the two X chromosomes.
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![[image-184.png|177x193]]
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Notes
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- This graph shows the purified neutrofils from 80 elderly (y >= 75) females
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-
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Fig 1. Distribution of X-chromosome inactivation patterns from peripheral blood of haematologically normal females in three different age groups |