Johan Dahlin
9b00451977
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32 lines
1.2 KiB
Markdown
32 lines
1.2 KiB
Markdown
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👋
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<small>The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.</small>
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<small>In other words, they studied human hematopoiesis acros s age by analyzing purified blood cell lineages from the subjects.</small>
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## Study
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- 197 hematologically normal females
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| | Sample size | Age | Median |
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| ----------- | ----------- | ----- | ------ |
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| Newborn | 23 | 0 | 0 |
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| Young adult | 94 | 17-50 | 30 |
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| Eldery | 80 | 75-96 | 81 |
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----
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### Methods
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- <small>Peripheral blood samples were collected</small>
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- <small>Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_</small>
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- <small>X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_</small>
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- <small>T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_</small > |