Johan Dahlin
434ce84982
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28 lines
1.1 KiB
Markdown
28 lines
1.1 KiB
Markdown
---
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date: 2021-12-16
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tags:
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- biokemi
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- provfråga
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- plasmid
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---
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**Plasmid DNA purified from colonies grown on blue/white screening plates was verified by electrophoresis. Samples in Lanes C and D are digested by enzyme for verification whilst Lanes A and B are non-digested samples. Select the correct description/descriptions based on the gel picture below.**
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![[Pasted image 20251128080404.png]]
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Välj ett eller flera alternativ:
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- A: The band in Lane A that migrates the furthest is in supercoiled form and we can predict its size by a comparison with the size markers.
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- B: The sample in Lane C has the largest size of DNA because it displays the brightest intensity under UV light.
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- C: By comparing Lanes C and D, we can infer that the sample in Lane D is a plasmid with an insert DNA whilst Lane C is an empty vector.
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- D: By comparing Lanes A and B, we can infer that the sample in Lane B is a plasmid with an insert DNA whilst Lane A is an empty vector.
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```spoiler-block
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Rätt svar
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1. C
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2. D
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*(Flersvarsfråga; i originalbilden är första påståendet felmarkerat, de två sista rättmarkerade.)*
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```
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