Johan Dahlin
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50 lines
2.1 KiB
Markdown
50 lines
2.1 KiB
Markdown
The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.
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In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects.
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## Study
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- 1 97 hematologically normal females
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- 23 newborn
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- 94 young adult (median 30)
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- 80 eldery (over 75)
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- purified neutrofils
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- purified T-cells
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----
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### Metoder
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1. Peripheral blood samples were collected
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2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_
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3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_
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4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_
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---
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### Results
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1. X chromosome usage is similar in newborns and young adults.
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2. Neutrophils from elderly female individuals show strongly uneven X chromosome usage.
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3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion
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---
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### Cord blood neutrophils
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![[image-183.png|148x156]]
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<small>
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- Graph shows the distribution of X chromosome usage in 23 newborn
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- X-axis: % of cells using the less common X chromosome.
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- Y-axis: number of individuals in each group.
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Conclusion: Newborns mostly show balanced use of the two X chromosomes
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</small>
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---
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### > 75 years neutrophils
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![[image-184.png|145x158]]
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<small>
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- This graph shows the purified neutrofils from 80 elderly (y >= 75) females
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- X and Y axis is the same as in previous graph
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Conclusion: Elderly neutrophils frequently show strongly uneven X chromosome usage.
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</small>
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----
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### Questions
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- What was the main question the authors wanted to answer?
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- Why did they choose this specific method instead of another one?
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- What does this figure show in one sentence?
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- What is the main conclusion from your article?
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- What would be the biggest limitation of this study?
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