Johan Dahlin
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21 lines
1.1 KiB
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21 lines
1.1 KiB
Markdown
<small>The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.</small>
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<small>In other words, they studied human hematopoiesis acros s age by analyzing purified blood cell lineages from the subjects.</small>
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---
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👋
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## Study
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- 197 hematologically normal females
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- 23 newborn - taken during delivery
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- 94 young adult - age: 17-50 median 30
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- 80 eldery - age: 75-96 median 81
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- purified neutrofils
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- purified T-cells
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----
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### Methods
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1. <small>Peripheral blood samples were collected</small>
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2. <small>Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_</small>
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3. <small>X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_</small>
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4. <small>T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_</small > |