vault backup: 2026-01-16 11:02:41

content/Vetenskaplig artikel/Methods & Main Results.md

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+The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.
+
+In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects.
+
 Modell/material
 - 197 hematologically normal females
 	- 23 new born
@@ -6,23 +10,12 @@ Modell/material
 - purified neutrofils
 - purified T-cells
 
-The model is _human hematopoiesis across age_ and the material is _lineage-purified blood cells_
+**Methods**
-
-methods:
-1. cell separation (neutrofil and T-cell)
-2. x-chromosome inactivation analysis (humana PCR)
-3. T-cell clonality check
-
-> [!NOTE]
-> They separated neutrophils and T cells, measured X-inactivation skewing, and checked that T cells were not dominating.
-
-### **What was compared / tested**
-
-- **Age groups:** newborn vs young adults vs elderly
-- **Cell lineages:** neutrophils vs T cells
-- **Readout:** degree of X-chromosome inactivation skewing
-
 
+1. Peripheral blood samples were collected
+2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_
+3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_
+4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_
 ### Results
 1. X chromosome usage is similar in newborns and young adults.
 2. Neutrophils from elderly individuals show strongly uneven X chromosome usage.