From c4bcca275aab620c83fae8b672b40a5790a2c601 Mon Sep 17 00:00:00 2001 From: Johan Dahlin Date: Fri, 16 Jan 2026 11:02:41 +0100 Subject: [PATCH] vault backup: 2026-01-16 11:02:41 --- .../Methods & Main Results.md | 25 +++++++------------ 1 file changed, 9 insertions(+), 16 deletions(-) diff --git a/content/Vetenskaplig artikel/Methods & Main Results.md b/content/Vetenskaplig artikel/Methods & Main Results.md index f41e4fb..de3df3d 100644 --- a/content/Vetenskaplig artikel/Methods & Main Results.md +++ b/content/Vetenskaplig artikel/Methods & Main Results.md @@ -1,3 +1,7 @@ +The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells. + +In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects. + Modell/material - 197 hematologically normal females - 23 new born @@ -6,23 +10,12 @@ Modell/material - purified neutrofils - purified T-cells -The model is _human hematopoiesis across age_ and the material is _lineage-purified blood cells_ - -methods: -1. cell separation (neutrofil and T-cell) -2. x-chromosome inactivation analysis (humana PCR) -3. T-cell clonality check - -> [!NOTE] -> They separated neutrophils and T cells, measured X-inactivation skewing, and checked that T cells were not dominating. - -### **What was compared / tested** - -- **Age groups:** newborn vs young adults vs elderly -- **Cell lineages:** neutrophils vs T cells -- **Readout:** degree of X-chromosome inactivation skewing - +**Methods** +1. Peripheral blood samples were collected +2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_ +3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_ +4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_ ### Results 1. X chromosome usage is similar in newborns and young adults. 2. Neutrophils from elderly individuals show strongly uneven X chromosome usage.