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<small>The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.</small> ---
aliases:
<small>In other words, they studied human hematopoiesis acros s age by analyzing purified blood cell lineages from the subjects.</small> ---
--- ---
👋 👋
---
<small>The aim of the study was to investigate whether normal aging leads to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.</small>
<small>In other words, they studied human hematopoiesis acros s age by analyzing purified blood cell lineages from the subjects.</small>
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## Study ## Study
- 197 hematologically normal females - 197 hematologically normal females
- 23 newborn - taken during delivery
- 94 young adult - age: 17-50 median 30
- 80 eldery - age: 75-96 median 81 | | Sample size | Age | Median |
- purified neutrofils | ----------- | ----------- | ----- | ------ |
- purified T-cells | Newborn | 23 | 0 | 0 |
| Young adult | 94 | 17-50 | 30 |
| Eldery | 80 | 75-96 | 81 |
---- ----
### Methods ### Methods
1. <small>Peripheral blood samples were collected</small> - <small>Peripheral blood samples were collected</small>
2. <small>Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_</small> - <small>Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_</small>
3. <small>X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_</small> - <small>X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_</small>
4. <small>T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_</small > - <small>T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_</small >