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2
content/.obsidian/core-plugins.json
vendored
2
content/.obsidian/core-plugins.json
vendored
@@ -22,7 +22,7 @@
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"random-note": false,
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"outline": true,
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"word-count": true,
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"slides": false,
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"slides": true,
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"audio-recorder": false,
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"workspaces": false,
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"file-recovery": true,
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@@ -2,6 +2,8 @@ The aim of the study was to investigate whether normal aging leads to blood cell
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In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects.
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-----
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Modell/material
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- 197 hematologically normal females
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- 23 new born
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@@ -10,17 +12,24 @@ Modell/material
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- purified neutrofils
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- purified T-cells
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----
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**Methods**
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1. Peripheral blood samples were collected
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2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_
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3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_
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4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_
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---
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### Results
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1. X chromosome usage is similar in newborns and young adults.
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2. Neutrophils from elderly individuals show strongly uneven X chromosome usage.
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2. Neutrophils from elderly female individuals show strongly uneven X chromosome usage.
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3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion
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---
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### Figure
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![[image-183.png|177x186]]
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Notes
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