From 76492126cacd2f81adb75529097240840a2b51b8 Mon Sep 17 00:00:00 2001 From: Johan Dahlin Date: Fri, 16 Jan 2026 11:10:57 +0100 Subject: [PATCH] vault backup: 2026-01-16 11:10:57 --- content/.obsidian/core-plugins.json | 2 +- .../Vetenskaplig artikel/Methods & Main Results.md | 11 ++++++++++- 2 files changed, 11 insertions(+), 2 deletions(-) diff --git a/content/.obsidian/core-plugins.json b/content/.obsidian/core-plugins.json index 7f63f5e..a8f4fd8 100644 --- a/content/.obsidian/core-plugins.json +++ b/content/.obsidian/core-plugins.json @@ -22,7 +22,7 @@ "random-note": false, "outline": true, "word-count": true, - "slides": false, + "slides": true, "audio-recorder": false, "workspaces": false, "file-recovery": true, diff --git a/content/Vetenskaplig artikel/Methods & Main Results.md b/content/Vetenskaplig artikel/Methods & Main Results.md index de3df3d..74d7a9e 100644 --- a/content/Vetenskaplig artikel/Methods & Main Results.md +++ b/content/Vetenskaplig artikel/Methods & Main Results.md @@ -2,6 +2,8 @@ The aim of the study was to investigate whether normal aging leads to blood cell In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects. +----- + Modell/material - 197 hematologically normal females - 23 new born @@ -10,17 +12,24 @@ Modell/material - purified neutrofils - purified T-cells + +---- + **Methods** 1. Peripheral blood samples were collected 2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_ 3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_ 4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_ + +--- ### Results 1. X chromosome usage is similar in newborns and young adults. -2. Neutrophils from elderly individuals show strongly uneven X chromosome usage. +2. Neutrophils from elderly female individuals show strongly uneven X chromosome usage. 3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion +--- + ### Figure ![[image-183.png|177x186]] Notes