jdahlin/medical-notes
1
0
Fork 0
Code Actions Activity

vault backup: 2026-01-16 11:10:57
All checks were successful
Deploy Quartz site to GitHub Pages / build (push) Successful in 3m21s
Details

Browse Source
This commit is contained in:
Johan Dahlin
2026-01-16 11:10:57 +01:00
parent c4bcca275a
commit 76492126ca
2 changed files with 11 additions and 2 deletions
Download Patch File Download Diff File Expand all files Collapse all files

2
content/.obsidian/core-plugins.json vendored
View File

@@ -22,7 +22,7 @@
"random-note": false, "random-note": false,
"outline": true, "outline": true,
"word-count": true, "word-count": true,
"slides": false, "slides": true,
"audio-recorder": false, "audio-recorder": false,
"workspaces": false, "workspaces": false,
"file-recovery": true, "file-recovery": true,

11
content/Vetenskaplig artikel/Methods & Main Results.md
View File

@@ -2,6 +2,8 @@ The aim of the study was to investigate whether normal aging leads to blood cell
In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects. In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects.
-----
Modell/material Modell/material
- 197 hematologically normal females - 197 hematologically normal females
- 23 new born - 23 new born
@@ -10,17 +12,24 @@ Modell/material
- purified neutrofils - purified neutrofils
- purified T-cells - purified T-cells
----
**Methods** **Methods**
1. Peripheral blood samples were collected 1. Peripheral blood samples were collected
2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_ 2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_
3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_ 3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_
4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_ 4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_
---
### Results ### Results
1. X chromosome usage is similar in newborns and young adults. 1. X chromosome usage is similar in newborns and young adults.
2. Neutrophils from elderly individuals show strongly uneven X chromosome usage. 2. Neutrophils from elderly female individuals show strongly uneven X chromosome usage.
3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion 3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion
---
### Figure ### Figure
![[image-183.png|177x186]] ![[image-183.png|177x186]]
Notes Notes