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Johan Dahlin
2026-01-16 12:16:12 +01:00
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content/.obsidian/plugins/image-converter/image-converter-image-alignments.json vendored
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@@ -2,14 +2,14 @@
"Vetenskaplig artikel/Methods & Main Results.md": { "Vetenskaplig artikel/Methods & Main Results.md": {
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content/Vetenskaplig artikel/Methods & Main Results.md
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@@ -1,9 +1,7 @@
The aim of the study was to investigate whether normal aging lea. ds to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells. The aim of the study was to investigate whether normal aging lea. ds to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells.
In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects. In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects.
----- -----
## Study ## Study
- 1 97 hematologically normal females - 1 97 hematologically normal females
- 23 newborn - 23 newborn
@@ -11,27 +9,20 @@ In other words, they studied human hematopoiesis across age by analyzing purifie
- 80 eldery (over 75) - 80 eldery (over 75)
- purified neutrofils - purified neutrofils
- purified T-cells - purified T-cells
---- ----
### Metoder
**Methods**
1. Peripheral blood samples were collected 1. Peripheral blood samples were collected
2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_ 2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_
3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_ 3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_
4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_ 4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_
--- ---
### Results ### Results
1. X chromosome usage is similar in newborns and young adults. 1. X chromosome usage is similar in newborns and young adults.
2. Neutrophils from elderly female individuals show strongly uneven X chromosome usage. 2. Neutrophils from elderly female individuals show strongly uneven X chromosome usage.
3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion 3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion
--- ---
### Cord blood neutrophils ### Cord blood neutrophils
![[image-183.png|287x302]] ![[image-183.png|148x156]]
<small> <small>
- Graph shows the distribution of X chromosome usage in 23 newborn - Graph shows the distribution of X chromosome usage in 23 newborn
- X-axis: % of cells using the less common X chromosome. - X-axis: % of cells using the less common X chromosome.
@@ -43,7 +34,7 @@ Conclusion: Newborns mostly show balanced use of the two X chromosomes
### > 75 years neutrophils ### > 75 years neutrophils
![[image-184.png|261x285]] ![[image-184.png|145x158]]
<small> <small>
- This graph shows the purified neutrofils from 80 elderly (y >= 75) females - This graph shows the purified neutrofils from 80 elderly (y >= 75) females
- X and Y axis is the same as in previous graph - X and Y axis is the same as in previous graph