diff --git a/content/.obsidian/plugins/image-converter/image-converter-image-alignments.json b/content/.obsidian/plugins/image-converter/image-converter-image-alignments.json index 387c5b0..1b664d3 100644 --- a/content/.obsidian/plugins/image-converter/image-converter-image-alignments.json +++ b/content/.obsidian/plugins/image-converter/image-converter-image-alignments.json @@ -2,14 +2,14 @@ "Vetenskaplig artikel/Methods & Main Results.md": { "aca677ab589429f9db4b9295c7397939": { "position": "center", - "width": "287px", - "height": "302px", + "width": "148px", + "height": "156px", "wrap": false }, "d765982220ac8d6615dac423d5f86ecc": { "position": "center", - "width": "261px", - "height": "285px", + "width": "145px", + "height": "158px", "wrap": false } } diff --git a/content/Vetenskaplig artikel/Methods & Main Results.md b/content/Vetenskaplig artikel/Methods & Main Results.md index e2fcd27..cac592b 100644 --- a/content/Vetenskaplig artikel/Methods & Main Results.md +++ b/content/Vetenskaplig artikel/Methods & Main Results.md @@ -1,9 +1,7 @@ The aim of the study was to investigate whether normal aging lea. ds to blood cells being produced from fewer cell clones. This was examined by comparing which X chromosome the cells were using between different age groups and between neutrophils and T cells. - In other words, they studied human hematopoiesis across age by analyzing purified blood cell lineages from the subjects. ----- - ## Study - 1 97 hematologically normal females - 23 newborn @@ -11,27 +9,20 @@ In other words, they studied human hematopoiesis across age by analyzing purifie - 80 eldery (over 75) - purified neutrofils - purified T-cells - - ---- - -**Methods** - +### Metoder 1. Peripheral blood samples were collected 2. Neutrophils and T cells were isolated using cell separation techniques _(centrifugation followed by lineage-specific purification)_ 3. X-chromosome inactivation was analyzed using the HUMARA PCR assay _(PCR-based measurement of which X chromosome is active)_ 4. T cells were analyzed by PCR to check for clonal expansion _(T-cell receptor γ gene rearrangement analysis)_ - --- ### Results 1. X chromosome usage is similar in newborns and young adults. 2. Neutrophils from elderly female individuals show strongly uneven X chromosome usage. 3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion - --- - ### Cord blood neutrophils -![[image-183.png|287x302]] +![[image-183.png|148x156]] - Graph shows the distribution of X chromosome usage in 23 newborn - X-axis: % of cells using the less common X chromosome. @@ -43,7 +34,7 @@ Conclusion: Newborns mostly show balanced use of the two X chromosomes ### > 75 years neutrophils -![[image-184.png|261x285]] +![[image-184.png|145x158]] - This graph shows the purified neutrofils from 80 elderly (y >= 75) females - X and Y axis is the same as in previous graph