From ff5995b7f4e89f41b3b0d0cbc9a1651d831dbe74 Mon Sep 17 00:00:00 2001 From: Johan Dahlin Date: Fri, 28 Nov 2025 11:40:06 +0100 Subject: [PATCH] vault backup: 2025-11-28 11:40:06 --- .../Biokemi/Plasmidlabb/Instuderingsfrågor.md | 24 +++++++++++++++++++ 1 file changed, 24 insertions(+) create mode 100644 content/Biokemi/Plasmidlabb/Instuderingsfrågor.md diff --git a/content/Biokemi/Plasmidlabb/Instuderingsfrågor.md b/content/Biokemi/Plasmidlabb/Instuderingsfrågor.md new file mode 100644 index 0000000..d105c9a --- /dev/null +++ b/content/Biokemi/Plasmidlabb/Instuderingsfrågor.md @@ -0,0 +1,24 @@ +--- +tags: + - biokemi + - instuderingsuppgifter + - plasmid +--- +#### Name three applications of molecular cloning. +#### What is a plasmid? +#### Name the three main steps involved in recombinant DNA technology? +#### What is a restriction enzyme? +#### What are the bacteria used in your protocol? +#### Explain the calcium/phosphate (heat-shock) method and what it is used for. +#### How can we selectively grow bacteria that have taken up the plasmid? +#### Explain the principle behind blue and white screening and its purpose in this lab. + +#### What are the sites the plasmid contains that allow for this experiment? +#### What are the 3 compounds in the LB plates that allow for selection of bacteria with plasmid and the distinction of plasmids with and without the inserted gene? +#### What are the six steps in plasmid preparation and purification? +#### What is a restriction enzyme and why was it used in this experiment? +#### Explain the principle of Gel Electrophoresis, what is it for? +#### What´s the compound that allows for the visualization of the DNA in the gel? +#### How many times does BamHI cut the plasmid without the insert? +#### And the plasmid with the insert? +#### Explain the different plasmid conformations that exist. Förståelse Biokemi / Plasmidlabb / \ No newline at end of file