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Johan Dahlin
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content/Biokemi/Gamla tentor/2021-12-16/34.md
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---
date: 2021-12-16
tags:
- biokemi
- provfråga
- plasmid
---
**Plasmid DNA purified from colonies grown on blue/white screening plates was verified by electrophoresis. Samples in Lanes C and D are digested by enzyme for verification whilst Lanes A and B are non-digested samples. Select the correct description/descriptions based on the gel picture below.**
![[Pasted image 20251128080404.png]]
Välj ett eller flera alternativ:
- A: The band in Lane A that migrates the furthest is in supercoiled form and we can predict its size by a comparison with the size markers.
- B: The sample in Lane C has the largest size of DNA because it displays the brightest intensity under UV light.
- C: By comparing Lanes C and D, we can infer that the sample in Lane D is a plasmid with an insert DNA whilst Lane C is an empty vector.
- D: By comparing Lanes A and B, we can infer that the sample in Lane B is a plasmid with an insert DNA whilst Lane A is an empty vector.
Rätt svar
1. C
2. D
*(Flersvarsfråga; i originalbilden är första påståendet felmarkerat, de två sista rättmarkerade.)*