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content/Vetenskaplig artikel/Methods & Main Results.md

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+Modell/material
+- 197 hematologically normal females
+	- 23 new born
+	- 94 young adult (median 30)
+	- 80 eldery (over 75)
+- purified neutrofils
+- purified T-cells
+
+The model is _human hematopoiesis across age_ and the material is _lineage-purified blood cells_
+
+methods:
+1. cell separation (neutrofil and T-cell)
+2. x-chromosome inactivation analysis (humana PCR)
+3. T-cell clonality check
+
+> [!NOTE]
+> They separated neutrophils and T cells, measured X-inactivation skewing, and checked that T-cells were not dominating.
+
+### **What was compared / tested**
+
+- **Age groups:** newborn vs young adults vs elderly
+- **Cell lineages:** neutrophils vs T cells
+- **Readout:** degree of X-chromosome inactivation skewing
+
+
+### Results
+1. X chromosome usage is similar in newborns and young adults.
+2. Neutrophils from elderly individuals show strongly uneven X chromosome usage.
+3. T-cells show much less uneven X chromosome usage and no dominant clonal expansion
+
+### Figure
+![[image-183.png|177x186]] 
+Notes
+- This graph shows the distribution of X chromosome usage in 23 newborn females
+- X-axis: in % how uneven the X chromosome usage is
+	- 50% means perfectly (50% maternal / 50% paternal)
+	- 0/100% means all cells use the same chromosome
+- Y-axis: number of individuals in each group
+
+Conclusion: 
+- Newborns mostly show balanced use of the two X chromosomes.
+
+![[image-184.png|177x193]]
+Notes
+- This graph shows the purified neutrofils from 80 elderly (y >= 75) females
+- 
+
+
+
+Fig 1. Distribution of X-chromosome inactivation patterns from peripheral blood of haematologically normal females in three different age groups