vault backup: 2025-12-09 15:12:34

content/Biokemi/Plasmidlabb/Instuderingsfrågor.md

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+---
+tags:
+  - biokemi
+  - instuderingsuppgifter
+  - plasmid
+---
+#### Name three applications of molecular cloning. 
+#### What is a plasmid? 
+#### Name the three main steps involved in recombinant DNA technology? 
+#### What is a restriction enzyme? 
+#### What are the bacteria used in your protocol? 
+#### Explain the calcium/phosphate (heat-shock) method and what it is used for. 
+#### How can we selectively grow bacteria that have taken up the plasmid? 
+#### Explain the principle behind blue and white screening and its purpose in this lab.
+
+#### What are the sites the plasmid contains that allow for this experiment? 
+#### What are the 3 compounds in the LB plates that allow for selection of bacteria with plasmid and the distinction of plasmids with and without the inserted gene?
+#### What are the six steps in plasmid preparation and purification?
+#### What is a restriction enzyme and why was it used in this experiment? 
+#### Explain the principle of Gel Electrophoresis, what is it for? 
+#### What´s the compound that allows for the visualization of the DNA in the gel? 
+#### How many times does BamHI cut the plasmid without the insert? 
+#### And the plasmid with the insert? 
+#### Explain the different plasmid conformations that exist. Förståelse Biokemi / Plasmidlabb /