vault backup: 2025-12-09 15:12:34

content/Biokemi/Gamla tentor/2021-12-16/34.md

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+---
+date: 2021-12-16
+tags:
+  - biokemi
+  - provfråga
+  - plasmid
+---
+**Plasmid DNA purified from colonies grown on blue/white screening plates was verified by electrophoresis. Samples in Lanes C and D are digested by enzyme for verification whilst Lanes A and B are non-digested samples. Select the correct description/descriptions based on the gel picture below.**
+
+![[Pasted image 20251128080404.png]]
+
+Välj ett eller flera alternativ:
+
+- A: The band in Lane A that migrates the furthest is in supercoiled form and we can predict its size by a comparison with the size markers.
+- B: The sample in Lane C has the largest size of DNA because it displays the brightest intensity under UV light.
+- C: By comparing Lanes C and D, we can infer that the sample in Lane D is a plasmid with an insert DNA whilst Lane C is an empty vector.
+- D: By comparing Lanes A and B, we can infer that the sample in Lane B is a plasmid with an insert DNA whilst Lane A is an empty vector.
+
+Rätt svar
+1. C
+2. D
+
+*(Flersvarsfråga; i originalbilden är första påståendet felmarkerat, de två sista rättmarkerade.)*