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Johan Dahlin
2025-11-30 01:06:30 +01:00
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content/Biokemi/Gamla tentor/2023-12-18/36.md
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@@ -10,6 +10,7 @@ tags:
![[Pasted image 20251129235214.png]]
**Answer**
```spoiler-block
The blue samples are samples with no DNA-insert.
They have an intact lacZ-gene and are able to produce β-galactosidase which breaks down X-gal to a blue pigment.
@@ -19,4 +20,5 @@ The reason that the blue cut shows two bands can be because a mixed colony was c
This could likely be the case because the second band has a 1500 bp size which the second band of the "white cut" also has.
Another possible reason for this odd result is contamination.
The "blue cut" sample could possibly have been contaminated with white cut samples when pipetting or by improper labelling of tubes.
This could be the case as the samples in both lanes show similar sizes (even the larger band is approximately the same size in both, 3000 bp).
This could be the case as the samples in both lanes show similar sizes (even the larger band is approximately the same size in both, 3000 bp).
```